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Addgene inc murine per2 promoter

Endogenous and temperature-compensated clock gene expression in mouse and reindeer fibroblast culture. (a) Representative luminescence data of mouse and reindeer fibroblasts under 37 °C ambient temperature. Expression of the Bmal1:luc reporter (black) is in approximate antiphase to the expression of <t>Per2:luc</t> reporter (gray) in both mouse and reindeer fibroblast cultures. Bioluminescence was measured in counts per minute (cpm). Phase angle difference (Ψ) between Bmal1:luc and Per2:luc for the first day after DEX synchronization (from hour 24 to hour 48) are plotted as circular plots. Here, the Bmal1 :luc phase is calculated relative to the Per2 :luc phase which was set as 0° and expressed as a proportion of τ. Data are normalized for the respective free-running period (τ). (b) Half-life times of Bmal1:luc expression under different ambient temperatures. Half-life times were calculated by damped sine wave analysis and are defined as the amount of time for the amplitude to dampen by half. (c) Periods of Bmal1:luc expression under different ambient temperatures. Periods have been determined by damped sine wave analysis. (d) Q10 values of Bmal1:luc in mouse and reindeer fibroblast cultures. Q10 values are based on periods as shown in panel c. Both Q10 values are within the acceptable range for temperature compensation (0.8-1.4) and do not differ significantly from each other (ns).

Murine Per2 Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews

murine per2 promoter - by Bioz Stars, 2026-06

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1) Product Images from "The Reindeer Circadian Clock Is Rhythmic and Temperature-compensated But Shows Evidence of Weak Coupling Between the Secondary and Core Molecular Clock Loops"

Article Title: The Reindeer Circadian Clock Is Rhythmic and Temperature-compensated But Shows Evidence of Weak Coupling Between the Secondary and Core Molecular Clock Loops

Journal: Journal of Biological Rhythms

doi: 10.1177/07487304241283066

Figure Legend Snippet: Endogenous and temperature-compensated clock gene expression in mouse and reindeer fibroblast culture. (a) Representative luminescence data of mouse and reindeer fibroblasts under 37 °C ambient temperature. Expression of the Bmal1:luc reporter (black) is in approximate antiphase to the expression of Per2:luc reporter (gray) in both mouse and reindeer fibroblast cultures. Bioluminescence was measured in counts per minute (cpm). Phase angle difference (Ψ) between Bmal1:luc and Per2:luc for the first day after DEX synchronization (from hour 24 to hour 48) are plotted as circular plots. Here, the Bmal1 :luc phase is calculated relative to the Per2 :luc phase which was set as 0° and expressed as a proportion of τ. Data are normalized for the respective free-running period (τ). (b) Half-life times of Bmal1:luc expression under different ambient temperatures. Half-life times were calculated by damped sine wave analysis and are defined as the amount of time for the amplitude to dampen by half. (c) Periods of Bmal1:luc expression under different ambient temperatures. Periods have been determined by damped sine wave analysis. (d) Q10 values of Bmal1:luc in mouse and reindeer fibroblast cultures. Q10 values are based on periods as shown in panel c. Both Q10 values are within the acceptable range for temperature compensation (0.8-1.4) and do not differ significantly from each other (ns).

Techniques Used: Gene Expression, Expressing

Temperature entrainment of clock genes expression in mouse and reindeer fibroblast cultures. Expression of Per2:luc and Bmal1:luc in mouse (a-c) and reindeer fibroblasts (d-f) under constant and cycling ambient temperature (Ta). Two sets of cultures were DEX-synchronized 12 h apart from each other. Bioluminescence was measured in counts per minute (cpm). For reindeer, bioluminescence data after the temperature cycle were plotted in zoomed-in plots besides the corresponding graphs. Times of peak expressions (center of gravity) were determined with the CircWave program for each rhythm to track phase relationships and responses before, during, and after the temperature cycle (c, f). Phase relationships between the initially 12 h-apart synchronized cell cultures of the same gene were calculated after the temperature cycle and tested with an unpaired t test (asterisk indicates p < 0.05, ns denotes p > 0.05). (g, h) Relative gene expressions of Bmal1 and Per2 in mouse and reindeer fibroblasts under one temperature cycle. Gene expression data were measured by qPCR with species-specific primers.

Figure Legend Snippet: Temperature entrainment of clock genes expression in mouse and reindeer fibroblast cultures. Expression of Per2:luc and Bmal1:luc in mouse (a-c) and reindeer fibroblasts (d-f) under constant and cycling ambient temperature (Ta). Two sets of cultures were DEX-synchronized 12 h apart from each other. Bioluminescence was measured in counts per minute (cpm). For reindeer, bioluminescence data after the temperature cycle were plotted in zoomed-in plots besides the corresponding graphs. Times of peak expressions (center of gravity) were determined with the CircWave program for each rhythm to track phase relationships and responses before, during, and after the temperature cycle (c, f). Phase relationships between the initially 12 h-apart synchronized cell cultures of the same gene were calculated after the temperature cycle and tested with an unpaired t test (asterisk indicates p < 0.05, ns denotes p > 0.05). (g, h) Relative gene expressions of Bmal1 and Per2 in mouse and reindeer fibroblasts under one temperature cycle. Gene expression data were measured by qPCR with species-specific primers.

Techniques Used: Expressing, Gene Expression

In mouse fibroblasts, the Bmal1:luc reporter entrains gradually to an ambient temperature cycle eventually resuming its approximate antiphase relationship with Per2 expression, suggesting that the Bmal1 -specific temperature effect is dominantly mediated via the circadian clock. In reindeer fibroblasts, Bmal1 is acutely responding to temperature cycles and moves into phase with Per2 expression. We suggest that a weak coupling between circadian loops reduces the effect of temperature via the RORE elements on the Bmal1 promoter; instead, an unknown RORE-independent pathway drives Bmal1 expression during temperature cycles in reindeer fibroblasts. Supporting this model, when we experimentally decoupled the circadian clock from the Bmal1:luc reporter by mutations of the ROREs, we rendered the promoter reporter acutely temperature-sensitive which led to a reindeer-like phase response under temperature cycles.

Figure Legend Snippet: In mouse fibroblasts, the Bmal1:luc reporter entrains gradually to an ambient temperature cycle eventually resuming its approximate antiphase relationship with Per2 expression, suggesting that the Bmal1 -specific temperature effect is dominantly mediated via the circadian clock. In reindeer fibroblasts, Bmal1 is acutely responding to temperature cycles and moves into phase with Per2 expression. We suggest that a weak coupling between circadian loops reduces the effect of temperature via the RORE elements on the Bmal1 promoter; instead, an unknown RORE-independent pathway drives Bmal1 expression during temperature cycles in reindeer fibroblasts. Supporting this model, when we experimentally decoupled the circadian clock from the Bmal1:luc reporter by mutations of the ROREs, we rendered the promoter reporter acutely temperature-sensitive which led to a reindeer-like phase response under temperature cycles.

Techniques Used: Expressing

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Journal: Journal of Biological Rhythms

Article Title: The Reindeer Circadian Clock Is Rhythmic and Temperature-compensated But Shows Evidence of Weak Coupling Between the Secondary and Core Molecular Clock Loops

doi: 10.1177/07487304241283066

Figure Lengend Snippet: Endogenous and temperature-compensated clock gene expression in mouse and reindeer fibroblast culture. (a) Representative luminescence data of mouse and reindeer fibroblasts under 37 °C ambient temperature. Expression of the Bmal1:luc reporter (black) is in approximate antiphase to the expression of Per2:luc reporter (gray) in both mouse and reindeer fibroblast cultures. Bioluminescence was measured in counts per minute (cpm). Phase angle difference (Ψ) between Bmal1:luc and Per2:luc for the first day after DEX synchronization (from hour 24 to hour 48) are plotted as circular plots. Here, the Bmal1 :luc phase is calculated relative to the Per2 :luc phase which was set as 0° and expressed as a proportion of τ. Data are normalized for the respective free-running period (τ). (b) Half-life times of Bmal1:luc expression under different ambient temperatures. Half-life times were calculated by damped sine wave analysis and are defined as the amount of time for the amplitude to dampen by half. (c) Periods of Bmal1:luc expression under different ambient temperatures. Periods have been determined by damped sine wave analysis. (d) Q10 values of Bmal1:luc in mouse and reindeer fibroblast cultures. Q10 values are based on periods as shown in panel c. Both Q10 values are within the acceptable range for temperature compensation (0.8-1.4) and do not differ significantly from each other (ns).

Article Snippet: The Per2:luc reporter vector (pLV6- Per2 -luc) consists of the backbone of the Bmal1:luc reporter vector (pLV6-Bmal-luc) and a murine Per2 promoter with adjacent luciferase sequence which is contained in the pGL3 basic E2 vector (Addgene 48747, a gift from Joseph Takahashi).

Techniques: Gene Expression, Expressing

Journal: Journal of Biological Rhythms

Article Title: The Reindeer Circadian Clock Is Rhythmic and Temperature-compensated But Shows Evidence of Weak Coupling Between the Secondary and Core Molecular Clock Loops

doi: 10.1177/07487304241283066

Figure Lengend Snippet: Temperature entrainment of clock genes expression in mouse and reindeer fibroblast cultures. Expression of Per2:luc and Bmal1:luc in mouse (a-c) and reindeer fibroblasts (d-f) under constant and cycling ambient temperature (Ta). Two sets of cultures were DEX-synchronized 12 h apart from each other. Bioluminescence was measured in counts per minute (cpm). For reindeer, bioluminescence data after the temperature cycle were plotted in zoomed-in plots besides the corresponding graphs. Times of peak expressions (center of gravity) were determined with the CircWave program for each rhythm to track phase relationships and responses before, during, and after the temperature cycle (c, f). Phase relationships between the initially 12 h-apart synchronized cell cultures of the same gene were calculated after the temperature cycle and tested with an unpaired t test (asterisk indicates p < 0.05, ns denotes p > 0.05). (g, h) Relative gene expressions of Bmal1 and Per2 in mouse and reindeer fibroblasts under one temperature cycle. Gene expression data were measured by qPCR with species-specific primers.

Techniques: Expressing, Gene Expression

Journal: Journal of Biological Rhythms

Article Title: The Reindeer Circadian Clock Is Rhythmic and Temperature-compensated But Shows Evidence of Weak Coupling Between the Secondary and Core Molecular Clock Loops

doi: 10.1177/07487304241283066

Figure Lengend Snippet: In mouse fibroblasts, the Bmal1:luc reporter entrains gradually to an ambient temperature cycle eventually resuming its approximate antiphase relationship with Per2 expression, suggesting that the Bmal1 -specific temperature effect is dominantly mediated via the circadian clock. In reindeer fibroblasts, Bmal1 is acutely responding to temperature cycles and moves into phase with Per2 expression. We suggest that a weak coupling between circadian loops reduces the effect of temperature via the RORE elements on the Bmal1 promoter; instead, an unknown RORE-independent pathway drives Bmal1 expression during temperature cycles in reindeer fibroblasts. Supporting this model, when we experimentally decoupled the circadian clock from the Bmal1:luc reporter by mutations of the ROREs, we rendered the promoter reporter acutely temperature-sensitive which led to a reindeer-like phase response under temperature cycles.

Techniques: Expressing

( A ) Differentiation protocol of miPSCs to chondrocytes. miPSCs were subjected to high-density micromass culture to create PDiPSCs. PDiPSCs were then either cast in agarose or pelleted and cultured in chondrogenic media for 14 to 21 days to create tissue-engineered cartilage. ( B ) P2L bioluminescence intensity (BLI) of miPSCs ( n = 10), PDiPSCs ( n = 2), PDiPSCs in agarose ( n = 4), and pellets ( n = 10) (left) and B1L bioluminescence intensity (BLI) of miPSCs ( n = 5), PDiPSCs ( n = 4), PDiPSCs in agarose ( n = 5) (middle), and pellets ( n = 5) (right). ( C ) BLI of femoral head cartilage explants from PER2::LUC mice ( n = 3, one hip per mouse). ( D ) P2L BLI in porcine chondrocytes cast in agarose ( n = 5). Shaded region on graphs represents SEM.

Journal: Science Advances

Article Title: Synthetic gene circuits for preventing disruption of the circadian clock due to interleukin-1–induced inflammation

doi: 10.1126/sciadv.abj8892

Figure Lengend Snippet: ( A ) Differentiation protocol of miPSCs to chondrocytes. miPSCs were subjected to high-density micromass culture to create PDiPSCs. PDiPSCs were then either cast in agarose or pelleted and cultured in chondrogenic media for 14 to 21 days to create tissue-engineered cartilage. ( B ) P2L bioluminescence intensity (BLI) of miPSCs ( n = 10), PDiPSCs ( n = 2), PDiPSCs in agarose ( n = 4), and pellets ( n = 10) (left) and B1L bioluminescence intensity (BLI) of miPSCs ( n = 5), PDiPSCs ( n = 4), PDiPSCs in agarose ( n = 5) (middle), and pellets ( n = 5) (right). ( C ) BLI of femoral head cartilage explants from PER2::LUC mice ( n = 3, one hip per mouse). ( D ) P2L BLI in porcine chondrocytes cast in agarose ( n = 5). Shaded region on graphs represents SEM.

Article Snippet: Two luciferase reporter lentiviral vectors, one driven by the murine Bmal1 promoter (Addgene no. 182761) and one driven by the murine Period 2 (Per2) promoter (Addgene no. 182762), were used ( ).

Techniques: Cell Culture

Per2 expression in cartilage explants from the femoral head of PER2::LUC mice was recorded as bioluminescence intensity. Explants were treated with IL-1β (1 ng/ml), IL-1⍺ (1 ng/ml), TNF⍺ (20 ng/ml), or vehicle controls ( n = 3 per each condition). Loss of circadian rhythm was seen in explants, given IL-1 and circadian rhythm was rescued with the administration of dex. Gray, blue, orange, and red bars indicate administration of PBS or cytokine; black bars indicate administration of dex. Shaded region on graph represents SEM. Asterisks represent significance compared to controls ( P < 0.05). ** P < 0.01; *** P < 0.05.

Journal: Science Advances

Article Title: Synthetic gene circuits for preventing disruption of the circadian clock due to interleukin-1–induced inflammation

doi: 10.1126/sciadv.abj8892

Figure Lengend Snippet: Per2 expression in cartilage explants from the femoral head of PER2::LUC mice was recorded as bioluminescence intensity. Explants were treated with IL-1β (1 ng/ml), IL-1⍺ (1 ng/ml), TNF⍺ (20 ng/ml), or vehicle controls ( n = 3 per each condition). Loss of circadian rhythm was seen in explants, given IL-1 and circadian rhythm was rescued with the administration of dex. Gray, blue, orange, and red bars indicate administration of PBS or cytokine; black bars indicate administration of dex. Shaded region on graph represents SEM. Asterisks represent significance compared to controls ( P < 0.05). ** P < 0.01; *** P < 0.05.

Techniques: Expressing

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1) Product Images from "The Reindeer Circadian Clock Is Rhythmic and Temperature-compensated But Shows Evidence of Weak Coupling Between the Secondary and Core Molecular Clock Loops"

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